ChondroArt je Educellov prvi celični terapevtski pripravek, ki vsebuje suspenzijo avtolognih hondrocitov in je namenjen zdravljenju hrustančnih poškodb. Tekom mnogih let nenehnega razvoja se je izdelek ChondroArt izboljševal tako po učinkovitosti zdravljenja kot tehniki pridobivanja celic in njihove uporabe. Izdelek 4. generacije, imenovan ChondroArt MSC, vsebuje mezenhimske matične celice, pridobljene iz aspirata kostnega mozga za zdravljenje kostno-hrustančnih in hrustančnih poškodb. Do danes je bilo z izdelki ChondroArt zdravljenih več kot 500 bolnikov.
ChondroArt
Generacije pripravkov
ChondorArt 1D: suspenzija avtolognih hondrocitov, pridobljenih iz hialinega hrustanca
ChondorArt 2D: avtologni hialini hondrociti, nasajeni na kolagenski nosilec
ChondroArt 3D: avtologni hialini hondrociti v hidrogelu iz alginata in agaroze
ChondroArt MSC: koncentrat aspirata kostnega mozga, bogat z avtolognimi mezenhimskimi matičnimi celicami, nasajenimi na biomimetični celični nosilec
Ortopedija: Pripravek ChondroArt MSC vsebuje kombinacijo mezenhimskih matičnih celic in biomimetičnega nosilca, kar omogoča zadrževanje implantiranih celic na mestu hrustančne/kostno-hrustančne poškodbe. Za vsaditev celičnega nosilca je potreben manjši operativni poseg (mini artrotomija).
Educellove znanstvene objave
Treatment of Pulmonary Sarcoidosis Using Allogenic Bone Marrow-Derived Mesenchymal Stem Cell Therapy is Safe: A Case Report
Pavelić E, Matišić V, Molnar V, Brlek P, Pavlovic T, Strbad M, Girandon L, Knežević M, Primorac D.
Int J Med Sci Clin Res. 2022 Jun; 2(6): 512-515.
Perinatal derivatives application: Identifying possibilities for clinical use
Gindraux F, Hofmann N, Agudo-Barriuso M, Antica M, Couto PS, Dubus M, Forostyak S, Girandon L, et. al.
Front Bioeng Biotechnol. 2022 Oct 11; 10: 977590.
Autologous Mesenchymal Stem Cells for Treatment of Chronic Active Antibody-Mediated Kidney Graft Rejection: Report of the Phase I/II Clinical Trial Case Series
Večerić-Haler Ž, Sever M, Kojc N, Halloran PF, Boštjančič E, Mlinšek G, Oblak M, Poženel P, Švajger U, Hartman K, Kneževič M, Barlič A, Girandon L, et al.
Transpl Int. 2022 Nov 22; 35: 10772.
Intraoperative intradermal application of stromal vascular fraction into the abdominal suture line: histological analysis of abdominal scar tissue
Živec K, Veber M, Pizem J, Jež M, Božikov K, Švajger U.
Aesthetic Plastic Surgery. 2022 Dec; 46: 2853–2862.
Compassionate mesenchymal stem cell treatment in a severe COVID-19 patient: a case report
Primorac D, Stojanović Stipić S, Strbad M, Girandon L, Barlič A, Frankić M, Ivić I, Marasović Krstulović D, Jukić I, Halassy B, Hećimović A, Matišić V, Molnar V.
Croat Med J. 2021 May; 62.
Autologous chondrocytes versus filtered bone marrow mesenchymal stem/stromal cells for knee cartilage repair-a prospective study
Martinčič D, Leban J, Filardo G, Busacca M, Barlič A, Veber M, Drobnič M.
Int Orthop. 2021 Apr;45(4):931-939.
Intracellular delivery of trehalose renders mesenchymal stromal cells viable and immunomodulatory competent after cryopreservation
Dovgan B, Miklavčič D, Knežević M, Zupan J, Barlič A.
Cytotechnology. 2021 Apr; 73 (3):1–21.
Human mesenchymal stromal cells from different tissues exhibit unique responses to different inflammatory stimuli
Burja B, Barlič A, Erman A, Mrak-Poljšak K, Tomšič M, Sodin-Semrl S, Lakota K.
Curr Res Transl Med. 2020 Nov; 68(4):217-224.
Combination of Filtered Bone Marrow Aspirate and Biomimetic Scaffold for the Treatment of Knee Osteochondral Lesions: Cellular and Early Clinical Results of a Single Centre Case Series
Veber M, Vogler J, Knežević M, Barlič A, Drobnič M.
Tissue Eng Regen Med. 2020 Jun; 17(3): 375–386.
Me2SO- and serum-free cryopreservation of human umbilical cord mesenchymal stem cells using electroporation-assisted delivery of sugars
Mutsenko V, Barlič A, Pezić T, Dermol-Černe J, Dovgan B, Sydykov B, Wolkers WF, Katkov II, Glasmacher B, Miklavčič D, Gryshkov O.
Cryobiology. 2019 Dec; 91:104-114.
Survival Rates of Various Autologous Chondrocyte Grafts and Concomitant Procedures. A Prospective Single-Center Study over 18 Years
Martinčič D, Mekač J, Drobnič M.
Cell Transplant. 2019 Nov; 28(11): 1439–1444.
Hematopoietic stem cell and mesenchymal stem cell population size in bone marrow samples depends on patient’s age and harvesting technique
Rebolj K, Veber M, Drobnič M, Maličev E.
Cytotechnology. 2018 Dec; 70(6): 1575–1583.
Treatment of Severe Steroid-Refractory Acute-Graft-vs.-Host Disease With Mesenchymal Stem Cells-Single Center Experience
Česen Mazič M, Girandon L, Kneževič M, Avčin SL, Jazbec J.
Front Bioeng Biotechnol. 2018 Jul 24;6:93.
Gene expression of cultured human chondrocytes as a model for assessing neutralization efficacy of soluble TNFα by TNFα antagonists.
Barlič A, Žigon S, Blejec A, Kregar Velikonja N
Biologicals. 2015 May;43(3):171-80.
Testing the potency of anti-TNF-α and anti-IL-1β drugs using spheroid cultures of human osteoarthritic chondrocytes and donor-matched chondrogenically differentiated mesenchymal stem cells
Žigon-Branc S, Barlič A, Knežević M, Jeras M, Vunjak-Novakovic G.
Biotechnol Prog. 2018 Jul; 34(4): 1045–1058.
Mesenchymal Stem Cells in the Musculoskeletal System: From Animal Models to Human Tissue Regeneration?
Čamernik K, Barlič A, Drobnič M, Marc J, Jeras M, Zupan J.
Stem Cell Rev Rep. 2018 Jun;14(3):346-369.
Pro-myogenic and low oxygen culture increases expression of contractile smooth muscle markers in human fibroblasts
Veber M, Dolivo D, Rolle M, Dominko T.
J Tissue Eng Regen Med. 2018 Mar; 12(3): 572-582.
Mineralization potential of cellulose-nanofibrils reinforced gelatine scaffolds for promoted calcium deposition by mesenchymal stem cells
Gorgieva S, Girandon L, Kokol V.
Mater Sci Eng C Mater Biol Appl. 2017 Apr 1;73:478-489.
Nanocellulose-Based Interpenetrating Polymer Network (IPN) Hydrogels for Cartilage Applications
Naseri N, Deepa B, Mathew AP, Oksman K, Girandon L.
Biomacromolecules. 2016 Nov 14;17(11):3714-3723.
Adipose-Derived Stem Cells Respond to Increased Osmolarities
Potočar U, Hudoklin S, Kreft ME, Završnik J, Božikov K, Fröhlich M.
PLoS One. 2016 Oct 5;11(10): e0163870.
Cryopreservation of Human Adipose-Derived Stem Cells in Combination with Trehalose and Reversible Electroporation
Dovgan B, Barlič A, Knežević M, Miklavčič D.
J Membr Biol. 2017 Feb;250(1):1-9.
Ten-year clinical and radiographic outcomes after autologous chondrocyte implantation of femoral condyles
Martinčič D, Radosavljevič D, Drobnič M.
Knee Surg Sports Traumatol Arthrosc. 2014 Jun;22(6):1277-83.
Cell sources for nucleus pulposus regeneration
Kregar Velikonja N, Urban J, Fröhlich M, Neidlinger-Wilke C, Kletsas D, Potočar U, Turner S, Roberts S.
Eur Spine J 23, 364–374 (2014).
Effect of intra-articular local anesthesia on articular cartilage in the knee
Ravnihar K, Barlič A, Drobnič M.
Arthroscopy. 2014 May;30(5):607-12.
Primary human alveolar bone cells isolated from tissue samples acquired at periodontal surgeries exhibit sustained proliferation and retain osteogenic phenotype during in vitro expansion
Marolt D, Rode M, Kregar-Velikonja N, Jeras M, Knezevic M.
PLoS One. 2014 Mar 25;9(3): e92969.
In vitro models for adipose tissue engineering with adipose-derived stem cells using different scaffolds of natural origin
Girandon L, Kregar-Velikonja N, Božikov K, Barlič A.
Folia Biol (Praha). 2011;57(2):47-56.
Cartilage from the edge of a debrided articular defect is inferior to that from a standard donor site when used for autologous chondrocyte cultivation
Malicev E, Barlič A, Kregar-Velikonja N, Stražar K, Drobnič M.
J Bone Joint Surg Br. 2011 Mar;93(3):421-6.
Bone tissue engineering with human stem cells
Marolt D, Knezevic M, Novakovic GV.
Stem Cell Res Ther. 2010 May 4;1(2):10.
Comparison of articular and auricular cartilage as a cell source for the autologous chondrocyte implantation
Malicev E, Kregar-Velikonja N, Barlič A, Alibegović A, Drobnic M.
J Orthop Res. 2009 Jul;27(7):943-8.
Growth and differentiation of alveolar bone cells in tissue-engineered constructs and monolayer cultures
Malicev E, Marolt D, Kregar Velikonja N, Kreft ME, Drobnic M, Rode M.
Biotechnol Bioeng. 2008 Jul 1;100(4):773-81.
Quantitative analysis of gene expression in human articular chondrocytes assigned for autologous implantation
Barlic A, Drobnic M, Malicev E, Kregar-Velikonja N.
J Orthop Res. 2008 Jun;26(6):847-53.
Tissue formation following implantation of cultured elastic chondrocytes for treatment of vesicoureteral reflux
Kregar-Velikonja N, Coer A, Gorensek M, Knezević M, Kmetec A.
Nephrol Dial Transplant. 2008 Feb;23(2):764-6.
Nucleus pulposus repair with cultured autologous elastic cartilage derived chondrocytes
Gorensek M, Jaksimović C, Kregar-Velikonja N, Gorensek M, Knezevic M, Jeras M, Pavlovcic V, Cör A.
Cell Mol Biol Lett. 2004;9(2):363-73.
The outcome of autologous chondrocyte transplantation treatment of cartilage lesions in the knee
Drobnic M, Kregar-Velikonja N, Radosavljevic D, Gorensek M, Koritnik B, Malicev E, Wozniak G, Jeras M, Knezevic M.
Cell Mol Biol Lett. 2002; 7(2):361-3.