Influencing chondrocyte differentiation phenotype ´in vitro´ by 3D scaffolds

Co-financed from the Slovenian Research Agency (code: L7-7598)

 

The project “Effect of 3D scaffolds on the differentiation ability of chondrocytes ´in vitro´” has just been finished. The main purpose of this project was to make a tissue engineering construct from a three dimensional gel scaffold, seeded with cells that have the correct chondrocyte phenotype expressed prior to implantation. Hydrogels based on alginate, alginate-agarose and other materials have been used, all of which present an environment that stimulates the native morphology of cartilage cells and is linked to higher expression of the desired proteins. By using hydrogels that simulate the native cartilage cell environment we wanted to slow down the process of dedifferentiation and, on the other hand, to speed up the redifferentiation of ´in vitro´ proliferated chondrocytes. At the same time this construct would allow less invasive surgical methods.